Lisa M. Landino
Professor of Chemistry
Ph.D. Department of Chemistry,
Graduate Advisor: Tim Macdonald
Fellow, Department of
Postdoctoral Advisor: Larry Marnett
Department of Chemistry
Office: Integrated Science Center 1283
Phone: (757) 221-2554
Advanced Freshmen Chemistry (Chem 205) and Gen. Chem I lab (Chem 103L)
Biochemistry (314) and Biochemistry lab (Chem 420)
My research focuses on oxidative damage to proteins and its role in neurodegeneration and aging. Oxidative damage contributes to the development of many diseases including Alzheimer’s and Parkinson’s. Currently, we are studying glycolytic enzymes especially pyruvate kinase and lactate dehydrogenase. The cysteines of these enzymes are particularly sensitive to damage by oxidants and modification by nitric oxide and glutathione.
All projects in the lab use the techniques of protein biochemistry including: column chromatography for protein purification, SDS-PAGE, Western blotting, labeling of proteins with fluorescent molecules, reverse phase HPLC, and UV/Visible spectroscopy.
Our past work focused on tubulin and its associated proteins including tau and MAP-2. Tau forms neurofibrillary tangles (NFTs) inside neurons that are a hallmark of Alzheimer’s disease.
NIH-AREA Award renewal (2R15 NS038885-05) “Peroxynitrite Damage to Microtubule Proteins” 7/1/11-8/31/16, $415,200 National Institute of Neurological Disorders and Stroke (NINDS).
Recent publications (undergraduate students underlined)
Lisa M. Landino, Tara D. Hagedorn and Kelly L. Kennett “Evidence for thiol/disulfide exchange reactions between tubulin and glyceraldehyde-3-phosphate dehydrogenase” Cytoskeleton (Wiley) 71 (2014), pp. 707-718.
Hillary M. Clark, Tara D. Hagedorn and Lisa M. Landino “Hypothiocyanous acid oxidation of tubulin cysteines inhibits microtubule polymerization” Archives of Biochemistry and Biophysics (Elsevier) 541C (2014), pp. 67-73.
Lisa M. Landino, Tara D. Hagedorn, Shannon B. Kim, Katherine M. Hogan Inhibition of tubulin polymerization by hypochlorous acid and chloramines, (2011) Free Radical Biology and Medicine 50, 1000-1008.
Lisa M. Landino, Carolyn M. Brown, Carolyn A. Edson, Laura J. Gilbert, Nathan Grega-Larson, Anna Jean Wirth, Kelly C. Lane, Fluorescein-labeled glutathione to study protein S-glutathionylation, (2010) Analytical Biochemistry 402, 102-104.
Lisa M. Landino (2008) Protein thiol modification by peroxynitrite anion and nitric oxide donors in Methods in Enzymology Nitric oxide Part F (Cadenas and Packer, Eds.) volume 440, pp. 95-109, Elsevier Inc. San Diego, CA.
Lisa M. Landino, Catherine B. Mall, Joshua J. Nicklay, Sarah K. Dutcher and Katherine L. Moynihan, Oxidation of 5-thio-2-nitrobenzoic acid, by the biologically-relevant oxidants peroxynitrite anion, hydrogen peroxide and hypochlorous acid, (2008) Nitric Oxide 18, 11-18.
Lisa M. Landino, Maria T. Koumas, Courtney E. Mason, Jane A. Alston Modification of tubulin cysteines by nitric oxide and nitroxyl donors alters tubulin polymerization activity, (2007) Chem. Res. Toxicol.20 (11), 1693-1700.